Table 3. List of primers and conditions used in qPCR for quantification of mRNAs.
Gene | Sequence Primer | Annealing Temperature | Amplicon Size |
---|---|---|---|
HTR2B | Forward: 5´ggatgcggttaaaagagaa 3´ Reverse: 5´ ccaattgccctcttgacaat 3´ |
65°C | 102 bp |
FADS2 | Forward: 5´ aatcagcaggggtttcaaga 3´ Reverse: 5´ ggcactacgctggagaagat 3´ |
65°C | 92 bp |
HIF-3α | Forward: 5´ gaatgggtctgcgagagtgt 3 Reverse: 5´ cccagtcggagagtatcgtc 3´ |
63°C | 108 bp |
UGDH | Forward: 5´ ttcctcgacaggattctacca 3´ Reverse: 5´ cagggtaacggttgttgatgt 3´ |
60°C | 110 bp |
ACO1 | Forward: 5´caatggctcagcaaggtgt 3´ Reverse: 5´ctgcttgggtcaggttcg 3´ |
64°C | 108 bp |
SYT16 | Forward: 5´cgccagagctgttggtgggg 3´ Reverse: 5´tgggctgaccacgccgaatg 3´ |
60°C | 192 bp |
SOD3 | Forward: 5´cttctgtattctgctattggttattc 3´ Reverse: 5´atgctgttctatgccccattctg 3´ |
60°C | 183 bp |