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. 2018 Apr 3;9(25):17349–17367. doi: 10.18632/oncotarget.24693

Table 3. List of primers and conditions used in qPCR for quantification of mRNAs.

Gene Sequence Primer Annealing Temperature Amplicon Size
HTR2B Forward: 5´ggatgcggttaaaagagaa 3´
Reverse: 5´ ccaattgccctcttgacaat 3´
65°C 102 bp
FADS2 Forward: 5´ aatcagcaggggtttcaaga 3´
Reverse: 5´ ggcactacgctggagaagat 3´
65°C 92 bp
HIF-3α Forward: 5´ gaatgggtctgcgagagtgt 3
Reverse: 5´ cccagtcggagagtatcgtc 3´
63°C 108 bp
UGDH Forward: 5´ ttcctcgacaggattctacca 3´
Reverse: 5´ cagggtaacggttgttgatgt 3´
60°C 110 bp
ACO1 Forward: 5´caatggctcagcaaggtgt 3´
Reverse: 5´ctgcttgggtcaggttcg 3´
64°C 108 bp
SYT16 Forward: 5´cgccagagctgttggtgggg 3´
Reverse: 5´tgggctgaccacgccgaatg 3´
60°C 192 bp
SOD3 Forward: 5´cttctgtattctgctattggttattc 3´
Reverse: 5´atgctgttctatgccccattctg 3´
60°C 183 bp