Table 1. Levels of expression of RANTES and IL-6 in conditioned media from stable transfectants.
| Cell line | RANTES (pg/48 h/105 cells) (mean ± SEM) |
P value | IL-6 (pg/48 h/105 cells) (mean ± SEM) |
P value |
|---|---|---|---|---|
| MCF-7EVz | 3.44 ± 0.06 | 1.06 ± 0.04 | ||
| MCF-7IL6 | 11.09 ± 0.12 | 0.0003 | 1574.36 ± 26.97 | 0.0003 |
| MCF-7EVn | 2.47 ± 0.29 | 0.80 ± 0.025 | ||
| MCF-7RANTES | 55.42 ± 1.41 | 0.0007 | 0.17 ± 0.039 | 0.0055 |
| MCF-7EVn+z | 4.53 ± 0.03 | 0.60 ± 0.20 | ||
| MCF-7RANTES+IL6 | 19.75 ± 0.43 | 0.0008 | 517.37 ± 8.80 | 0.0003 |
| MDA-MB-231EVz | 0.41 ± 0.007 | 1273.01 ± 40.69 | ||
| MDA-MB-231IL6 | 0.52 ± 0.002 | 0.0111 | 1690.70 ± 2.49 | 0.0094 |
| MDA-MB-231EVn | 0.52 ± 0.005 | 1003.23 ± 21.54 | ||
| MDA-MB-231RANTES | 161.76 ± 12.93 | 0.0063 | 849.57 ± 22.70 | 0.0391 |
| MDA-MB-231EVn+z | 0.55 ± 0.31 | 808.35 ± 19.76 | ||
| MDA-MB-231RANTES+IL6 | 163.35 ± 5.02 | 0.0009 | 1908.05 ± 34.54 | 0.0013 |
EV = Empty vector; n = neomycin; z = zeocin.
values were referred to 1 × 105 cells as determined on the harvesting day, 48 h after seeding. The data presented above were obtained from three independent measurements. The P values were calculated with the Student’s t-test (cells overexpressing RANTES or/and IL-6 versus cells transfected with their respective empty vectors).