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. 2018 Apr 3;9(25):17564–17575. doi: 10.18632/oncotarget.24786

Figure 3. Effect of sulforaphane on the protein expression of cathepsin S of SCC-9 and SCC-14 cells.

Figure 3

(A) The cell protein lysate was collected and detected the protein expression by Human Proteinases Array. (B) The expressions of cathepsin S protein on the treatments of SCC-9 and SCC-14 cells were assessed by western blot. The values represented the means ± SD of at least 3 independent experiments. (C–D) SCC-9 and SCC-14 cells transfected with the siRNA of cathepsin S for 48 h and analyzed by western blot and Boyden chamber migration assay. (E–F). The cells were treated with cathepsin S inhibitor Z-FL-COCHO (20 μM) for 24 h then analyzed by western blot and Boyden chamber migration assay. The values represented the mean ± S.D. from 3 determinations per condition repeated 3 times. *p < 0.05, compared with the vehicle group.