Table 2. List of antibodies used for immunohistochemical analysis.
| Antibody | Species (dilution) | 2nd antibody | Evaluation | |
|---|---|---|---|---|
| ER | SP1, Ventana Medical Systems, Tucson, USA | rabbit monoclonal (prediluted) |
Ventana iVIEW DAB Detection Kit | percentage of cells showing positive nuclear staining |
| PgR | 1E2, Ventana Medical Systems, Tucson, USA | rabbit monoclonal (prediluted) |
Ventana iVIEW DAB Detection Kit | percentage of cells showing positive nuclear staining |
| HER2 | 4B5, Ventana Medical Systems, Tucson, USA | rabbit monoclonal (prediluted) |
Ventana iVIEW DAB Detection Kit | 0, 1+, 2+, 3+ |
| Ki67 | MIB-1, DAKO, Glostrup, Denmark |
mouse monoclonal (1:200) |
Dako EnVison FLEX system | labeling index |
| AR | AR27, Novocastra, Newcastle, UK |
mouse monoclonal (1:50) |
Dako EnVison FLEX system | percentage of cells showing positive nuclear staining |
| VDR | NBP1-19478, Novus, Littleton, USA |
rabbit polyclonal (1:500) |
Dako EnVison FLEX system | percentage of cells showing positive nuclear staining |
| AKT phospho-Ser473 (pAKT) | D9E, Cell Signaling, Beverly, USA |
rabbit monoclonal (1:50) |
Dako EnVison FLEX system | percentage of cells and intensity showing positive cytoplasmic staining |
| ERα phospho-Ser167 (pER) | GTX50140, GeneTex, Irvine, USA |
rabbit polyclonal (1:50) |
Dako EnVison FLEX system | percentage of cells showing positive nuclear staining |