Figure 1. TS differentially induces the expression CXCR3⁺ Tregs in periphery and CRC Tumors IL-10 knockout mice on a C57BL/6 background were given 100 ml of vehicle or TS (100 mg/kg body weight) every 3 days by oral gavage from 8 weeks after the onset of symptomatic colitis through week 16.

The cells from LPLs were stained for FoxP3 and CXCR3 gated on CD4 T cells, then analyzed by flow cytometry. Changes in the mean percentage of FoxP3+CXCR3+ cells are shown in upper right quadrants (A). Azoxymethane (AOM) and Dextran sodium sulphate (DSS) induced BL/6 mice were given similar dose of TS for three weeks and tumor were isolated and stained for Foxp3 and CXCR3 gated on CD8 T cells (B). Experimental groups consisted of 5 mice; experiments were repeated three times.