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. 2017 Sep 1;125(9):097001. doi: 10.1289/EHP1443

Figure 1.

Figure 1A, 1F, and 1G are bar graphs with standard errors of mean plotting total time of chemoinvestigation, total number, and total duration in seconds, respectively, (y-axis) for the Veh and 0.5, 5, and 50 micrograms per kilogram per day DEHP groups (x-axis). Figures 1B and 1C are bar graphs with standard errors of mean plotting number of arm entries and percent time spent with the stimulus, respectively, for male and female components of the exposure groups (x-axis). Figures 1D and 1E illustrate sound waveforms (y-axis) for the duration of exposure to for Vehicle and DEHP-50), respectively, in seconds (x-axis).

Effects of phthalate di(2-ethylhexyl) phthalate (DEHP) on olfactory preference and courtship vocalizations in male mice. (A) Total time spent in the chemoinvestigation of male (♂) and sexually receptive female (♀) stimuli by males exposed to the vehicle (Veh) or DEHP (0.5, 5, or 50μg/kg/d). (B) Number of entries into the male or female arm of the Y-maze. (C) Percentage of time spent in investigating males (♂) vs. sexually receptive females (♀). Data are expressed as the means ±SEM of 11–12 males per treatment group, *p<0.05 vs. the male arm. (D–E) Representative sound waveform for males exposed to vehicle (D) or DEHP at 50μg/kg/d (E) in the presence of a sexually receptive female. (F–G) Total number (F) and duration (G) of ultrasonic vocalizations (USVs) produced during the 4-min test by males exposed to the vehicle or DEHP at 0.5, 5, or 50μg/kg/d. Data are expressed as the means ±SEM of 11–12 males per treatment group; *p<0.05, **p<0.01.