Table 2.

List of differentially expressed proteins identified by the proteomic analysis.

Spot ID	All groups	DEHP-5/Vehicle		DEHP-50/Vehicle		DEHP-50/DEPH-5		Accession number	Protein name	Gene name	Mascot score	Number of unique peptides	Sequence coverage (%)
	p-Value	p-Value	Ratio	p-Value	Ratio	p-Value	Ratio
e0035	0 .0013	0.0153	1.6	0.2748	$-1.2$	0.0003	$-1.9$	P56480	ATP synthase subunit beta mitochondrial	Atp5b	464.9	7	9.3
e0011	0.0155	0.0407	1.4	0.2496	$-1.2$	0.0107	$-1.7$	A8DUK4	Beta-globin	Hbbt1	153.4	2	17.0
0292	0.0115	0.1072	$-1.3$	0.0963	1.2	0.0090	1.6	Q8CAQ8	Mitochondrial inner membrane protein	Immt	469.4	8	12.8
1226	0.0371	0.2880	1.1	0.1185	$-1.2$	0.0211	$-1.4$	P56480	ATP synthase subunit beta mitochondrial	Atp5b	93.3	2	4.3
0348	0.0275	0.1685	$-1.2$	0.1579	1.2	0.0143	1.4	P20029	78 kDa glucose-regulated protein	Hspa5	1134.6	17	31.5
0484	0.0079	0.0822	$-1.2$	0.0634	1.1	0.0083	1.3	P63038	60 kDa heat shock protein mitochondrial	Hspd1	612.4	10	19.9
								P46660	Alpha-internexin	Ina	492.0	8	13.6
								P42932	T-complex protein 1 subunit theta	Cct8	490.0	7	15.0
1273	0.0329	0.9353	1.0	0.0134	1.3	0.0390	1.3	P17742	Peptidyl-prolyl cis-trans isomerase A	Ppia	433.6	10	39.0
e0571	0.0257	0.1106	$-1.2$	0.2886	1.1	0.0103	1.3	Q60864	Stress-induced-phosphoprotein 1	Stip1	604.3	14	27.1
e0051	0.0691	0.1661	$-1.2$	0.0302	$-1.3$	0.3220	$-1.1$	P14152	Malate dehydrogenase cytoplasmic	Mdh1	147.7	3	10.8
0656	0.0467	0.3558	1.3	0.0075	1.7	0.1530	1.3	P03995	Glial fibrillary acidic protein	Gfap	1175.2	18	37.9
1328	0.2000	0.0486	1.4	0.3277	1.2	0.4250	$-1.1$	Q9ER97	Neuroglobin	Ngb	160.5	3	22.5
0743	0.0746	0.0801	1.4	0.8369	1.0	0.0242	$-1.4$	P60710	Actin cytoplasmic 1	Actb	262.0	7	16.8
e0562	0.1622	0.2589	1.4	0.0379	1.5	0.6510	1.1	P03995	Glial fibrillary acidic protein	Gfap	677.6	10	24.7
e0603	0.1293	0.5013	1.2	0.0113	1.4	0.2740	1.2	Q9Z1N5	Spliceosome RNA helicase Ddx39b	Ddx39b	273.6	5	10.0
0434	0.0161	0.0142	$-1.3$	0.9955	1.0	0.0121	1.3	O08553	Dihydropyrimidinase-related protein 2	Dpysl2	671.9	12	21.0
0476	0.0197	0.3031	1.1	0.0141	1.3	0.0596	1.2	P61979	Heterogeneous nuclear ribonucleoprotein K	Hnrnpk	442.8	7	18.6
0771	0.0125	0.0221	$-1.3$	0.2263	$-1.1$	0.0325	1.2	Q9JHI5	Isovaleryl-CoA dehydrogenase mitochondrial	Ivd	533.7	10	23.3
0798	0.0298	0.0166	1.3	0.4885	1.1	0.0452	$-1.2$	P05063	Fructose-bisphosphate aldolase C	Aldoc	362.2	6	16.8
								P63085	Mitogen-activated protein kinase 1	Mapk1	302.4	7	16.8
0921	0.0127	0.5274	1.1	0.0183	$-1.3$	0.0100	$-1.5$	P06151	L-lactate dehydrogenase A chain	Ldha	332.2	6	18.4
1355	0.0124	0.0217	1.3	0.4772	$-1.1$	0.0123	$-1.4$	P19536	Cytochrome c oxidase subunit 5B mitochondrial	Cox5b	72.0	2	8.6

Note: Statistical tests and protein identification results from the 2D-DIGE analysis. The accession numbers are the protein identifiers in the UniProt database (http://www.uniprot.org). The Mascot score threshold for protein identification by liquid chromotagraphy tandem-mass spectrometry (LC-MS/MS) was 31 per peptide ($p<0.05$), and a minimum of two significant peptides was required to consider the protein identified. APT, adenosine triphosphate; ID, identifier.