FIG. 6.

Modulation of immune effector cell accumulation in RM1 prostate tumors by IL-27 sonoporation (Sono-27) in vivo. (A) Increased accumulation of CD3⁺CD8⁺ T cells was observed in RM1 tumors treated with four sonoporation treatments as described in Materials and Methods. Left: Flow cytometry data. Right: The percent change observed over control (tumors sonoporated with control empty plasmid). *p<0.009. (B) Decreased accumulation of Gr1⁺CD11b⁺CD124⁺ cells (p<0.03) was observed in Sono-27-treated RM1 tumors, and proportions of Gr1⁺CD11b⁺ cells (p<0.002) were also reduced. (C) Decreased accumulation of CD4⁺CD25⁺FoxP3⁺ cells (p<0.001) was observed in Sono-27-treated RM1 tumors. Color images available online at www.liebertonline.com/hum