FIG. 7.

Modulation of immune effector cell accumulation in TRAMP-C2 prostate tumors by IL-27 sonoporation or Ad therapy in vivo. (A) Increased accumulation of CD3⁺CD8⁺ T cells was observed in TRAMP-C2 tumors treated with sonoporation IL-27 therapy (Sono-27; p<0.03) as described in Materials and Methods. In sharp contrast, in Ad-27-treated tumors, a reduction in CD3⁺CD8⁺ was observed instead (p<0.04). Presented is the percent change observed for each treatment group as compared with the respective control groups. (B) Reduced accumulation of MDSC populations was observed for both Sono-27- and Ad-27-treated TRAMP-C2 tumors. Sono-27 reduced accumulation of Gr1⁺CD11b⁺CD124⁺ cells as a trend (p=0.13) and reduced Gr1⁺CD11b⁺ cells (p<0.03), whereas Ad-27 reduced Gr1⁺CD11b⁺CD124⁺ (p<0.006) and Gr1⁺CD11b⁺ populations (p<0.03). (C) Reduced accumulation of potential Treg population. Both Sono-27 and Ad-27 reduced CD4⁺CD25⁺FoxP3⁺ populations (p<0.03) in treated TRAMP-C2 tumors.