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Effect of NEM and I-Ac modification on ATP hydrolysis and synthesis
| Treatment | Rate of ATP Hydrolysis | Rate of ATP Synthesis |
|---|---|---|
| μmol Pi × mg Chl−1 h−1 | μmol ATP × mg Chl−1 h−1 | |
| None | 27 | 1,390 |
| 2 mm NEM | 202 | 1,625 |
| 10 mm I-Ac | 218 | 1,525 |
Thylakoids were first reduced by incubation with 50 mm diothiothreitot (DTT) and then alkylated by either NEM or I-Ac as described in “Materials and Methods.” “None” refers to thylakoids that had not been incubated with DTT or an alkylating reagent.
