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. Author manuscript; available in PMC: 2018 Apr 24.
Published in final edited form as: Cell Rep. 2018 Mar 27;22(13):3480–3492. doi: 10.1016/j.celrep.2018.03.002

Figure 4. Expression of EZH2 Opposes Entry into OIS and Induction of SASP.

Figure 4

(A) LF1 cells were engineered using lentivirus vectors to stably express EZH2 cDNA and a 4-OHT-inducible ER:RAS(G12V) protein. Control cell lines were constructed using empty vectors. Cells were treated with 4-OHT (200 nM) or vehicle (ethanol) for the indicated times, and the levels of EZH2 and ER:RAS proteins were determined by immunoblotting.

(B) Proliferation in the experiment shown in (A) was assessed by counting cell numbers (*p < 0.05, **p < 0.01, n = 3).

(C) SA-β-Gal-positive cells were scored after 12 days of 4-OHT treatment in the experiment shown in (A) (**p < 0.01, n = 3).

(D) p16 mRNA expression was determined by real-time qPCR as in (C) (**p < 0.01, n = 3).

(E) Expression of the indicated SASP genes was determined by real-time qPCR as in (C) (*p < 0.05, **p < 0.01, n = 3).

Error bars represent SD. See also Table S1.