(A) Heat map of genes involved in BA signaling pathways in liver and ileum. The colors on the heatmap corresponded to the relative changes in average gene expression. Red represents up-regulation while green represents down-regulation. (2) QuantiGene Plex 2.0 Assay was performed to measure the gene expression of Fxr, Shp, Lrh-1 and Fgfr4 in the liver and Fxr, Shp, Fgf15, and Tgr5 in the ileum. Relative mRNA levels were calculated with vehicle controls set as 100%. Data represent means ± S.E.M. *p<0.05 versus vehicle treatment group; #p<0.05 versus WT group.