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. Author manuscript; available in PMC: 2018 Apr 24.
Published in final edited form as: Oncogene. 2015 Sep 14;35(18):2345–2356. doi: 10.1038/onc.2015.295

Figure 5. Restoration of expression of SiM-miRNAs depletes AR and steroid receptor coactivators in PC cells.

Figure 5

LNCaP cells were transfected with the indicated SiM-miRNA mimetics for 48 hours. Then, cells were harvested and total cell lysates were prepared. Immunoblot analyses were conducted for the expression levels of AR, SRC-1, SRC-2, SRC-3, p-AKT(S473), total AKT, p-S6(S235/236), total S6, cleaved PARP, c-Myc, SKP2 and β-Actin in the cell lysates. The numbers beneath the bands represent densitometry analysis of band intensity relative to the expression of β-Actin.