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. Author manuscript; available in PMC: 2018 Apr 24.
Published in final edited form as: Nat Protoc. 2017 Sep 7;12(10):2081–2096. doi: 10.1038/nprot.2017.093

Figure 7.

Figure 7

Using isosurfaces to make spatial measurements. Time-series montage shows 3D reconstructions at several time points over the course of AC invasion. The AC is expressing the PI(4,5)P2 sensor mCherry∷PLCδPH (cyan); the BM is labeled with LAM-1∷GFP (magenta). Isosurface renderings (green) were used to measure the volume of the AC’s invasive protrusion that had extended through the BM. Scale bar represents 5 μm.