Fig. 2. Electrophysiological analysis of infected mosquito primary neuron cultures.

a Spontaneous spiking activity with average ratio of total spike (TS) number per electrode between 0 dpi (reference) and TS number at 2 and 7 dpi of spontaneous activity. b Mosquito neurons stimulated activity with average ratio of TS number per electrode between gabazine stimulus and solvent (water) introduction; n = number of active electrodes. c 3D electrode activity maps of the effect of gabazine stimulation on mosquito neuronal networks for 2 min, post water as baseline and post gabazine. Statistical differences were calculated with unpaired t tests by comparing uninfected group to infected groups unless indicated by bracket. *P < 0.05; **P < 0.01; ***P < 0.001; means ± SEM. For (a), n values are detailed in Supplementary Table S2