Fig. 3.

SHARP1 is crucial for clonogenic growth of human MLL-AF6 AML cells. a Western blots of SHARP1 protein in ML-2, CTS and SHI-1 cells transduced with the indicated shRNAs. Western blots are representative of at least three independent experiments. b Kaplan–Meyer survival curve of sublethally irradiated recipient NSG mice transplanted with 5 × 10⁴ ML-2 or 1 × 10⁵ CTS cells transduced with the indicated shRNAs. P values are determined by the Log-rank (Mantle-Cox) Test. c Left panel: Representative flow cytometry plot of ML-2, CTS, and SHI-1 cells transduced with the indicated shRNAs for AnnexinV and DAPI or PI. Right panel: Percentage of AnnexinV⁺and DAPI⁻ or PI⁻ cell are shown. d Cell count of ML-2, CTS, and SHI-1 cells transduced with the indicated shRNAs in culture. The value is determined as fold increase in cell number relative to the number of cells initially plated. e Colony-forming units (CFU) of ML-2, CTS, and SHI-1 cells transduced with the indicated shRNAs. The number of colonies observed 7 days after the plating. The graphs are representative examples of three independent experiments and presented as mean ± s.e.m, *p < 0.05, ** p < 0.01, ***p < 0.001