Figure 7.
Design of experimental groups. (a) Mice were fed either a diet containing 10% fat (control mice) or 60% fat (obese mice) for 12 weeks. Non-ventilated control or obese mice (NVC and NVO) underwent general anesthesia, were intubated and were sacrificed after 5 min of spontaneous ventilation. (b) Mice were fed the same diet (10% or 60% fat). Ventilated control and obese mice underwent general anesthesia, were intubated and were sacrificed after 2 hours of mechanical ventilation, with a tidal volume calculated on actual weight for control mice (VC) or on either actual weight (OVTaw) or ideal weight (OVTiw) for obese mice.