Fig. 7.

A-ROD recruits EBP1 to the DKK1 promoter. a RNA recovery of A-ROD, DKK1 or GAPDH transcripts after RNA purification, using biotinylated antisense probes targeting A-ROD (specific) or lacZ (control), measured by RT-qPCR. b DNA recovery of the depicted loci measured by qPCR of DNA eluted from RNA purification using biotinylated antisense probes targeting A-ROD or lacZ. Error bars represent standard deviations from three independent experiments (n = 3 biological replicates). c Silver staining of SDS-PAGE analyzed proteins, eluted from RNA purification using biotinylated antisense A-ROD specific or lacZ control probes. Marked bands were excised, destained and subjected to mass spectrometry. d Western blot for HNRNPK and EBP1 on SDS-PAGE analyzed proteins, eluted from RNA purification using biotinylated antisense A-ROD specific or lacZ control probes. Anti-HNRNPK was first applied on the blot, followed by anti-EBP1. e ChIP for EBP1 in control and A-ROD siRNA treated MCF-7 cells. Error bars represent standard deviations from three independent experiments (n = 3 biological replicates, **p < 0.01, two-tailed Student’s t-test). f Normalized RT-qPCR RNA levels in control and EBP1 siRNA treated MCF-7 and MDA-MB-231 cells. Error bars represent standard deviations from three independent experiments (n = 3 biological replicates, **p < 0.01, two-tailed Student’s t-test)