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. 2018 Apr 18;9:388. doi: 10.3389/fphar.2018.00388

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Copyright © 2018 Zhang, den Braver-Sewradj, den Braver, Hiemstra, Vermeulen, van de Water, Commandeur and Vos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Concentrations of GSH-conjugates of AQ-QI (AQ-SG) and DEAQ-QI (DEAQ-SG) (A), AQ (B), and DEAQ (C) in total lysates of mock- and GSTP1-transfected HepG2 cells. When applicable, pre-exposure of 500 μM BSO was performed 24 h before treatment. Cells were treated with 50 μM AQ-QI or 30 μM DEAQ-QI for 2.5 h, after which GSH conjugates and reduces QIs were analyzed using HPLC. Results are presented as mean ± SD from four independent experiments.