Figure 3.

Preferential variants of exon 4 and exon 6 after bacterial stimulation in hemocyte. (A) Number of observed clones of exon 4 in hemocytes post-challenged with various bacteria and PBS (control). The variants of exon 4 were analyzed by cloning and sequencing 80 clones from each group. Individual colonies were randomly selected from each sample. All assays were repeated on two independent occasions, and values represent the mean number of observed clones from two independent experiments. The serial number of exon 4/6 was assigned and the amino-acid sequences of exon 4/6 are shown in Figure S3 in Supplementary Material. (B) Number of observed clones of exon 6 post-challenge with various bacteria and PBS. (C) Differential sequence conservation of epitopes I and II of EsDscam following alternative splicing of exon 4 and exon 6. (Upper panel) Sequence logo representation of the conservation of exon 4 variants in Eriocheir sinensis; (lower panel) sequence logo representation of the conservation of exon 6 variants in E. sinensis. Bits (y-axis) indicate units of evolutionary conservation.