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. 2018 Apr 18;10:116. doi: 10.3389/fnagi.2018.00116

FIGURE 1.

FIGURE 1

Autophagy negatively regulates NALP3 inflammasome activation in PrP106-126 treated microglial cells. LPS-primed microglial BV2 cells were stimulated with PrP106-126 (100 μM), the autophagy inhibitor, 3-MA (2 mM) and the autophagy inducer, rapamycin (100 nM). (A,B) Western blot of LC3-II in lysates of BV2 cells incubated with PrP106-126 and 3-MA for 6 h. (C,D) Western blot of LC3 II in lysates of BV2 cells treated with PrP106-126 and rapamycin for 6 h. (E,F) Western blot of NALP3 and ASC in lysates of BV2 cells treated with PrP106-126 and 3-MA for 6 h. (G,H) Western blot of NALP3 in lysates of LPS-primed BV2 cells treated with PrP106-126 and rapamycin for 6 h. (I–L) Western blot analysis of caspase-1, IL-1β, and ratio IL-1β/pro-IL-1βin lysates of BV2 cells treated with PrP106-126 and 3-MA for 12 h. (M) ELISA analysis of IL-1β in supernatants of BV2 cells treated with PrP106-126 and 3-MA for 12 h. (N–Q) Western blot of caspase-1, IL-1β, and ratio IL-1β/pro-IL-1β in lysates of primed BV2 cells treated with PrP106-126 and rapamycin for 12 h. (R) ELISA analysis of IL-1β in supernatants of BV2 cells treated with PrP106-126 and rapamycin for 12 h. Data represent mean ± SD of three separate experiments. ∗P < 0.05, ∗∗P < 0.01. Groups which are compared are shown in the graphs.