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. 2018 Apr 18;10:116. doi: 10.3389/fnagi.2018.00116

FIGURE 2.

FIGURE 2

Gene silencing of NALP3 inflammasome components augments autophagy. (A) Western blot analysis of NALP3 and ASC in lysates of NALP3 or ASC siRNA-transfected BV2 microglia cells. (B) Western blot analysis of LC3-II in lysates of NALP3 or ASC siRNA-transfected BV2 microglia cells with exposure to PrP106-126. (C,D) Western blot analysis of caspase-1 in lysates of NALP3 or ASC siRNA-transfected BV2 cells treated with PrP106-126 and 3-MA (2 nM) for 12 h. (E,F) ELISA analysis of IL-1β in supernatants of primed- and NALP3 or ASC siRNA-transfected BV2 cells treated with PrP106-126 and 3-MA (2 nM) for 12 h. Data represent mean ± SD of three separate experiments. ∗P < 0.05, ∗∗P < 0.01. Groups which are compared are shown in the graphs.