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. 2018 Apr 24;9:1628. doi: 10.1038/s41467-018-04063-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Gal-3 KO mice exhibited augmented IFN-γ production. a Quantification of IFN-γ, IL-21, IL-4, IL-6, and IL-2 by ELISA in the supernatants of purified CD4⁺ T cells from the spleen of WT and Gal-3 KO mice cultured with anti-CD3 and anti-CD28 for 72 h (n = 4 for each condition). b Representative plots and statistical analysis of the frequency of splenic IFN-γ-producing CD4⁺ T cells of WT and Gal-3 KO mice (n = 5 for both strains). c Representative plots and statistical analysis of the frequency of splenic IFN-γ-producing B220⁺ B cells of WT and Gal-3 KO mice (n = 5 for both strains). d Representative plots and statistical analysis of the frequency of splenic IFN-γ-producing non-Tfh, Tfh, and Tfr cells of Gal-3 KO mice (n = 5). e Representative plots and statistical analysis of the percentage of splenic IFN-γ-producing CD4⁺ T cells in the mixed BM chimeras shown in Fig. 3d containing an 80:20 ratio of μMT:Gal-3^+/+ WT or μMT:Gal-3 KO BM at 10 weeks after transplantation (μMT+Gal-3^+/+ n = 7; μMT+Gal-3^−/− B cells n = 7). f Representative plots and statistical analysis of the percentage of splenic IFN-γ-producing CD4⁺ T cells in the mixed BM chimeras shown in Fig. 3h containing a 70:30 ratio of CD45.1 Gal-3^+/+ WT: CD45.2 Gal-3 KO BM at 10 weeks after transplantation (n = 9). g Representative histograms and statistical analysis of splenic IFN-γR expression on non-GC and GC B cells and on CD4⁺ T cells of WT (dotted lines) Gal-3 KO mice (solid lines) (n = 5 for both strains). h Representative histograms and statistical analysis of T-bet expression in splenic non-GC B cells of WT (n = 5, dotted lines) and Gal-3 KO mice (n = 6, solid lines) and in GCs from Gal-3 KO mice. i Representative histograms and statistical analysis of T-bet expression in splenic non-Tfh CD4⁺ T cells of WT (n = 5, dotted lines) and Gal-3 KO mice (n = 6, solid lines) and in Tfh from Gal-3 KO mice. Data are the mean ± s.e.m. *p < 0.05, **p < 0.005, ***p < 0.001. ns, not significant (unpaired (a–e, g–i) and pair (f) Student’s t-test). a–i Data are representative of three independent experiments