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. 2018 Apr 18;9:769. doi: 10.3389/fmicb.2018.00769

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Copyright © 2018 Wang, Zhang, Liu, Xing and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MoUBP14 affects functions of appressorium. (A) Appressorium formation assay. Conidia were incubated on hydrophobicsurfaces and observed at different time points. Bar, 10 μm. (B) Appressorium formation rates at different time. Asterisks representsignificant differences (P < 0.05, n > 100). (C) Cytorrhysis assay for appressorium turgor pressure. Drops of conidial suspension (1 × 10⁵ conidia ml^-1) were placed on the hydrophobic surface of coverslips and treated with indicated concentration of PEG8000 at 24 hpi. (D) Transmission electron microscopy (TEM) observation of the appressorium. Melanin layer was indicated by arrows.