Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 24;8:6438. doi: 10.1038/s41598-018-24695-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Elf3 deficiency reduces collagenase activity in mice following destabilization of the medial meniscus (DMM). RT-qPCR analyses of total RNA isolated from articular cartilage from unoperated (Ctrl) and DMM-operated (DMM) joints of WT and cKO mice (n = 3/ea) at 8 weeks after surgery showing DMM-induced Elf3 mRNA in WT and cKO counterparts, represented as relative expression to Rpl13a (left) and fold-change vs. unoperated controls (right) (A). Nos2 and Mmp13 mRNA levels were assessed in cartilage isolated from DMM-operated limbs (n = 3/genotype) at 8 weeks after DMM. (B) Data is shown as fold change vs. WT levels (set at 1). (C) Representative images of unoperated (Control) or DMM-operated (8wk DMM) sections of WT and Elf3 cKO mice at 8 weeks after surgery stained with the C1, 2C antibody. (D) Quantification of the C1,2C-positive immunostaining at 8-weeks post-DMM revealed reduced collagenase activity in the cKO mice. Data are shown as fold-change over the immunopositive signal detected in the control knees, set at 1 (n = 3/ea). All data are shown as mean ± S.D. ***p < 0.001 by t-test (B), and *p < 0.05 and **p < 0.01 by ANOVA followed by Tukey’s post-hoc test (A and D). Comparisons with significant differences are indicated; all other comparisons were non-significant.