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. 2018 Apr 24;8:6434. doi: 10.1038/s41598-018-24710-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemistry using the AT8 antibody in hippocampal (a,c,e,g) and entorhinal cortex (b,d,f,h) sections from WT (a,b), WT_M (c,d), Tg2576 (e,f) and Tg2576_M (g,h) mice. Scale bars: 50 µm. Quantification of AT8 staining per hippocampal section (i). Data are the mean ± SEM of six different areas covering the entire hippocampus from two sections on the anatomically comparable plane for each animal (n = 3). Statistical significance was analysed by two-way ANOVA with Tukey’s post-hoc test. **p < 0.005.