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. 2018 Apr 24;8:6434. doi: 10.1038/s41598-018-24710-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of maternal transmission of non-genetic material on neuronal cytoskeletal markers. The levels of cytoskeleton markers in total protein cortical preparations WT, WT_M, Tg2576 and Tg2576_M mice were analysed by Western blot. Representative blot and quantification of immunolabeled bands for (a) Arc versus actin, (b) pCofilin versus actin and (c) MAP2 versus actin. Full-length blots are presented in Supplementary Figure 5. The results (fold over control –WT-) are expressed as the mean ± SEM (n = 4). Statistical significance was analysed by two-way ANOVA with Tukey’s post-hoc test. *p < 0.05, **p < 0.005, ***p < 0.0001 respect to WT; ^###p < 0.0001 respect to Tg2576.