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. Author manuscript; available in PMC: 2018 Apr 25.
Published in final edited form as: Methods Mol Biol. 2016;1422:263–280. doi: 10.1007/978-1-4939-3603-8_24

Table 4.

Collection tubes and histology cassettes required for gerbil dissection and tissue collection

Collection tube/cassette Purpose
Tube 1 Microcentrifuge tube for blood collection
Tube 2a Microcentrifuge tube with 1 mL of sterile 1× PBS with antifungal/antibiotics for gastric tissue collection for quantitative culturea
Tube 3 Freezer tube for gastric tissue collection for long-term storage at −80 °C
Cassette Histology cassette labeled with pencil or solvent-resistant marker for gastric tissue collection for histology
Tube 4 Microcentrifuge tube with 900 μL sterile 1× PBS with antifungal/antibiotics for 1:10 serial dilution
Tube 5 Microcentrifuge tube with 900 μL sterile 1× PBS with antifungal/antibiotics for 1:100 serial dilution
Tube 6 Microcentrifuge tube with 900 μL sterile 1× PBS with antifungal/antibiotics for 1:1000 serial dilution
a

Weigh tube 2 prior to collection of tissue to ultimately determine CFU per gram of gastric tissue.