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(A) Competitive ELISA using MAM-BSA conjugate (5 μg/ mL) as a coating antigen. The concentration of anti-MAM antibody was kept at 1 μg/ mL. The concentrations of analytes (heroin, monoacetyl morphine, morphine, and codeine) were prepared between 0-1000 ng/ mL. The IC50 values for opiate drugs were found to be 0.35, 0.30, 0.20, 0.21 ng/mL for heroin, MAM, morphine, and codeine respectively.
