Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 23;373(1748):20170337. doi: 10.1098/rstb.2017.0337

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Author(s)

Published by the Royal Society. All rights reserved.

PMC Copyright notice

Figure 3. — The m^N^4,5C residue disrupts normal DNA structure. (a) Structural considerations of the m^N^4,5C residue in dsDNA [20,36,37] show how rotation around the N4–C4 bond of m^N^4,5C relieves the steric clash between the methyl groups (upper panel) but at the same time disrupts the normal G · C hydrogen-bonding pattern (lower panel), precluding a normal Watson–Crick DNA structure. (b) Impact of m^N^4,5C on DNA structure probed by susceptibility to nuclease S1 digestion. pBR322 plasmid DNA containing m^N^4,5C, mono-methylated (either m^N⁴C or m⁵C) or unmethylated cytosine (c), respectively, at CC(A/T)GG sites (electronic supplementary material, figure S9) was incubated with nuclease S1 and analysed by agarose gel electrophoresis. R.MvaI lane, commercial pBR322 digested with R.MvaI; L, GeneRuler DNA Ladder Mix.