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. 2018 Apr 24;9(2):e00360-18. doi: 10.1128/mBio.00360-18

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Copyright © 2018 Wu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Interactions of FtsX and EnvC. (A) Functionality of a plasmid expressing Flag-tagged FtsX (X-FLAG) or EnvC (C-FLAG) in the ΔftsX or ΔenvC mutant was examined by phase-contrast microcopy. (B) Cell lysates of both strains in panel A were subjected to immunoblotting with antibodies against the Flag tag (anti-Flag [α-Flag]) with duplicate samples. (C) Cell membrane preparations from the ΔenvC mutant expressing C-FLAG were subjected to coimmunoprecipitation using IgG control (prebleed) or anti-FtsX antibodies. Protein samples derived from the input (before immunoprecipitation [IP]), IgG⁻, and anti-FtsX fractions were immunoblotted with anti-FtsX and anti-Flag antibodies. (D) Cells of the indicated strains were treated with formaldehyde (CH₂O) or mock treated. Protein samples were analyzed by Western blotting with α-Flag antibody.