H. pylori-EBV coinfection results in upregulation of EBV latent and lytic genes. (A) NCI-N87 cells were coinfected with H. pylori-EBV-GFP along with the control. Cells were examined for the expression of latent and lytic marker genes (EBNA1 and BZLF1, respectively) at different time points (1, 2, and 5 dpi) using fluorescence microscopy. DIC, differential interference contrast; DAPI, 4′,6-diamidino-2-phenylindole. (B to E) The expression profiles of EBNA1 (B), BZLF1 (C), EBNA3C (D), and gp350 (E) were analyzed at the transcript level using RT-qPCR.