TABLE 1 .
Binding constants (KD) determined in direct binding assays of CIRV p19 with different sRNAs and apparent dissociation constants (KC) determined in competitive binding assays with p19 bound to gf698 siRNA
| sRNA | KD (nM) | Relative KD | KC (nM) | Krel (normalized KC) |
|---|---|---|---|---|
| gf698 (siRNA) | 0.06 ± 0.04 | 1 | 0.07 ± 0.03 | 1 |
| AtmiR162, NbmiR162b | 0.07 ± 0.03 | 1.2 | 0.12 ± 0.05 | 1.7 |
| NbmiR162a | 0.14 ± 0.05 | 1.8 | NDa | ND |
| AtmiR168a, NbmiR168d,e | 2.29 ± 0.78 | 38.2 | 1.59 ± 0.35 | 22.7 |
| AtmiR168b | 4.83 ± 1.57 | 80.5 | ND | ND |
| NbmiR168a,b,c | 6.57 ± 1.50 | 109.5 | ND | ND |
| AtmiR403 | 1.37 ± 0.29 | 22.8 | 1.09 ± 0.43 | 15.6 |
| NbmiR403a | 0.11 ± 0.02 | 1.8 | ND | ND |
| NbmiR403b | 0.37 ± 0.06 | 6.2 | ND | ND |
a
ND, not determined.