Skip to main content
. 2018 Apr 24;200(10):e00048-18. doi: 10.1128/JB.00048-18

FIG 2.

FIG 2

Sequence alignment of the N-terminal regions of the ST2186 protein and homologous bacterial proteins. Alignment of amino acid sequences was performed using the ClustalW program provided from DDBJ. ST2186n and Ec_n indicate the nucleotide sequences corresponding to the N-terminal regions of the ST2186 protein and the GF6PAT protein from Escherichia coli (locus, AE005174), respectively. ST2186p, Hi, Bs, and Ec_p indicate the amino acid sequences of the N-terminal regions of the ST2186 protein and the GF6PAT proteins from Haemophilus influenzae (locus, AAC22088), Bacillus subtilis (locus, GLMS_BACPZ), and E. coli (locus, AE005604_9), respectively. The underlined codon is the start codon (ATG) used for construction of the expression vector for the ST2186L protein. The double-underlined minor start codon (GTG) was used for construction of the expression vector for the ST2186S protein after conversion to the ATG codon. Gaps are shown by dashes.