FIG 9.

Elution profiles of the substrate and the phosphorylated amino sugar molecules produced by the purified fraction and the recombinant ST2245 protein. Ion chromatography elution profiles of the product produced from GlcN-6-P by the recombinant ST2245 protein (line a), the product produced from GlcN-6-P by the final fraction of the native purified enzyme from the S. tokodaii cell extract (line b), and the standard GlcN-6-P substrate molecule (line c) are shown. The arrow indicates the product (GalN-6-P) peak produced from GlcN-6-P.