Phylogenetic reconstruction of a concatenation of H, D, and K subunits of nitrogenase and uncharacterized nitrogenase paralogs (n = 420 concatenated protein sequences). All nodes shown exhibited bootstrap supports of >90% (out of 1,000 bootstrap replicates), except where a black box (>70%) is shown. Nif, molybdenum (Mo) nitrogenase; Anf, iron-only (Fe) nitrogenase; Vnf, vanadium (V) nitrogenase; Unc, uncharacterized nitrogenase-like proteins.