FIG 5.

Verification of the Zur binding site in IutE and IutA. (A) Putative iutE and iutA Zur binding sites compared to the Actinobacteria Zur consensus site determined previously (41); many positions within the consensus are variable, and the most prominent bases are shown. (B) Relative positions of different promoter fragments for iutE and summary of the results shown in the EMSA with recombinant Zur (C). (C) EMSA with recombinant Zur incubated with the iutA promoter region and with different portions of the iutE promoter region. Recombinant Zur was added where indicated (+).