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. 2000 Sep;124(1):461–474. doi: 10.1104/pp.124.1.461

Table III.

Sulfate uptake and xylem loading of sulfate in excised roots from wild-type and transgenic poplar exposed to H2S

Wild Type Wild Type Transgenic Transgenic
0 μL L−1 H2S 0.25 μL L−1 H2S 0 μL L−1 H2S 0.25 μL L−1 H2S
Sulfate uptake (nmol g fresh wt−1 h−1) 129  ± 45a,A 118  ± 37a,A 152  ± 43a,B 164  ± 35a,B
Xylem loading (nmol g fresh wt−1 h−1) 8.5  ± 4.2b 4.1  ± 1.1a 10.0  ± 4.3b 8.1  ± 2.9b
Xylem loading (% of total uptake) 6.4  ± 2.5b 3.6  ± 0.9a 6.5  ± 1.7b 5.0  ± 1.6a

Excised poplar roots were pre-incubated in the incubation chamber described by Herschbach and Rennenberg (1991) with 0.1 mm sulfate for 2 h and subsequently exposed to 0.1 mm [35S]sulfate. After 4 h of incubation radioactivity in root segments and in the solution of the exudation compartment were measured by liquid scintillation counting. The data shown are means ± sd with seven poplar plants of each poplar line and each treatment. Significant differences between treatments at P < 0.05 are indicated with different small letters; differences between poplar lines are indicated with capital letters.