Table III.
Wild Type | Wild Type | Transgenic | Transgenic | |
---|---|---|---|---|
0 μL L−1 H2S | 0.25 μL L−1 H2S | 0 μL L−1 H2S | 0.25 μL L−1 H2S | |
Sulfate uptake (nmol g fresh wt−1 h−1) | 129 ± 45a,A | 118 ± 37a,A | 152 ± 43a,B | 164 ± 35a,B |
Xylem loading (nmol g fresh wt−1 h−1) | 8.5 ± 4.2b | 4.1 ± 1.1a | 10.0 ± 4.3b | 8.1 ± 2.9b |
Xylem loading (% of total uptake) | 6.4 ± 2.5b | 3.6 ± 0.9a | 6.5 ± 1.7b | 5.0 ± 1.6a |
Excised poplar roots were pre-incubated in the incubation chamber described by Herschbach and Rennenberg (1991) with 0.1 mm sulfate for 2 h and subsequently exposed to 0.1 mm [35S]sulfate. After 4 h of incubation radioactivity in root segments and in the solution of the exudation compartment were measured by liquid scintillation counting. The data shown are means ± sd with seven poplar plants of each poplar line and each treatment. Significant differences between treatments at P < 0.05 are indicated with different small letters; differences between poplar lines are indicated with capital letters.