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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Dev Biol. 2018 Mar 13;438(1):44–56. doi: 10.1016/j.ydbio.2018.03.004

Figure 2. atoh1c-expressing progenitors give rise to to ventral r1 and cerebellar granule neurons.

Figure 2

A–L: Tg(atoh1c∷kaede) transgene expression in fixed embryos stained with anti-Kaede antibody in dorsal (A,D,G,J), ventral (B,E,H,K) and lateral (C,F,I,L) views at 22 hpf (A–C), 2 dpf (D–F), 2 dpf (G–I) and 5 dpf (J–L). Arrowheads follow the color code described in Fig. 1 legend or are labeled as follows: PF: parallel fibers, r1: MHB-derived neurons in ventral r1. M–P: live imaging after photoconversion of Kaede (green to red) of MHB atoh1c+ cells at 22 hpf confirms that this Tg(atoh1c∷kaede)+ progenitor domain gives rise to ventral r1 neurons. Dorsal (M) and ventral focal planes (N–P). R–T: Tg(atoh1c∷kaede)+ cells (green) transiently express TH (red; gray arrowheads) from 22 hpf to 2 dpf and lie adjacent to the LC (indicated by white bracket). All images oriented with anterior to the left at time points as indicated. Scale bars: 50 μM.