Fig. 4.

S100A9 deficiency does not change survival or weight change after CLP, but does impair local bacterial clearance. There was no significant difference in survival after CLP in wild-type and S100A9^−/− mice (A, Gehan-Breslow-Wilcoxon test, p=0.5). S100A9^−/− mice lost and regained a similar percentage of baseline body weight after CLP as wild-type controls (B, 2-way ANOVA, p=0.0005 for effect of time, p=0.28 for effect of genotype). While there was no significant difference in intraperitoneal bacterial burden 3 days after CLP, S100A9^−/− mice failed to clear intraperitoneal infection 7 days after CLP (C, 2-way ANOVA p<0.01 for interaction of time and genotype, Sidak’s p=0.003 at 7 days for differences among genotypes). Low levels of disseminated bacteria were detected in the spleen (D, 2-way ANOVA p=0.35) and brain (E, 2-way ANOVA p=0.08) but did not differ by genotype. ** p <0.01