Figure 7. TC DCs present an intrinsic inflammatory phenotype.

Frequencies and numbers of total splenic DCs (A) and IL6⁺ DCs (B) in B6.Rag and TC.Rag mice, as compared to age-matched TC mice. C. IL-6 intracellular staining in splenic DCs from B6.Rag (black) and TC.Rag (white) 24 h after LPS or CpG stimulation. Cytokine gene expression in unstimulated, and secretion in supernatant by BMDCs from the four indicated strain before and after stimulation with LPS: IL-6 (D); IL-1b (E), and IL-33 (F). G. Il12b gene expression and protein expression in supernatants of BMDCs stimulated with LPS for 6 h (qRT-PCR) and 24 h (ELISA). H. Tnfa, and Il10 gene expression in BMDCs, without stimulation for Tnfa, and 6 h after LPS stimulation for Il10. Gene expression was normalized to the B6 mean values. # p < 0.05 one-tailed t test; * p < 0.05, ** p < 0.01, *** p < 0.001. n = 4–6 per strain.