Skip to main content
. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Cell Physiol. 2018 Feb 27;233(8):5684–5695. doi: 10.1002/jcp.26421

Figure 2.

Figure 2

Constructing a tropism-modified adenovirus (Ad.5/3) carrying the mda-7/IL-24 gene. The genome of Ad.5/3.mda-7 was generated by homologous recombination between the linearized plasmid pShCMV.mda-7 and 8st81-digested genomic DNA of Ad.5/3-Luc, and kanamycin selection resulted in the pAd.5/3-mda-7 genome, where the CMV promoter in place of the early viral El region drives mda-7/IL-24 expression. This plasmid was digested with Pad to release viral ITRs and transfected in A549 cells to rescue the Ad.5/3-mda-7. (Das et al., Adv. Cancer Res., 2012)