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. 2018 Apr 18;9:355. doi: 10.3389/fphys.2018.00355

Figure 4.

Figure 4

HG-induced endothelial protein O-GlcNAcylation and inflammation were reduced by OGT siRNA transfection. (A) OGT gene expression levels were reduced to 72% of the control level by OGT siRNA in HG-stimulated HAECs (HG+siOGT) N = 5. ***p < 0.001 compared with HG-treated HAECs transfected with negative control (HG+NC). (B) OGT protein expression levels were significantly reduced by OGT siRNA in HG-stimulated HAECs, as compared with HG+NC. The blot is representative of three independent experiments. (C) Protein O-GlcNAcylation levels were significantly reduced by OGT siRNA in the HG-stimulated HAECs, as compared with HG+NC. The blot is representative of three independent experiments. (D) siOGT transfection inhibited HG-induced endothelial ICAM-1 mRNA expression to 58%, VCAM-1 mRNA expression to 71%, E-selectin mRNA expression to 67% of the control level in the HG-stimulated HAECs N = 5. **p < 0.01 compared with HG+NC. (E) ICAM-1 expression levels were significantly reduced by OGT siRNA in HG-stimulated HAECs, as compared with HG+NC. The blot is representative of three independent experiments. (F) siOGT transfection inhibited HG-induced THP-1 adhesion to 24% of the control level in the HG-stimulated HAECs N = 3. ***p < 0.001 compared with HG+NC.