Figure 4.

Effects of manipulation of integrin αvβ5 activity on tumorigenesis. 3-dpi kras+ and WT larvae were treated cilengitide or ADP together with dox for 4 days. Liver size were measured and liver sections were stained for cell proliferation and apoptosis. More than 20 fish were analyzed in each group. (A) Gross morphology of larvae after 4 days of treatment (left lateral view). The WT livers were outlined. (B) Quantification of liver size. (C) IF staining of PCNA on liver sections. (D) Quantification of PCNA-positive cells in the liver. (E) IF staining of Caspase 3 on liver sections. (F) Quantification of Caspase 3-positive cells in the liver. Livers are marked by dash lines. Int indicates intestine. *P < .05. Scale bar: 20 μm.