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. 2018 Mar 21;20(5):411–424. doi: 10.1016/j.neo.2018.02.005

Figure 2.

Figure 2

POH1 knockdown inhibits cell proliferation. (A) The effect of POH1 siRNA was confirmed by Western blot analysis. (B) POH1 knockdown impaired QGY-7701, EC109, and HCT116 cell viability. Cells transfected with POH1 siRNA (siPOH1-1 or siPOH1-2) or scrambled siRNA were cultured in a 96-well plate for 4 days. Cell viability was determined using the MTT assay. (C) POH1 depletion weakened the colony formation ability of the above cell lines. The number of colonies is shown. (D) The effect of POH1 overexpression was confirmed by Western blot analysis. (E) POH1 overexpression failed to affect the viability of QGY-7701, EC109, and HCT116. Cells transfected with POH1 or vector were cultured in a 96-well plate for 4 days. Cell viability was determined using MTT assay. (F) POH1 overexpression failed to affect monoclonal formation ability of above cell lines. The number of colonies is shown. All *P<.05 and **P<.01.