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. 2018 Apr 5;20(5):499–509. doi: 10.1016/j.neo.2018.03.001

Figure 1.

Figure 1

Pathogenic Salmonella decreases Wnt1 protein expression in host cells.

(A) Wnt1 protein expression in human HCT116 cells. Cells were incubated with wild-type S. typhimurium (SL1314) for 30 minutes, washed, and incubated in fresh DMEM with 10% FBS for 15, 30, or 60 minutes or 2 hours. Total cell lysates were analyzed for total Wnt1 levels by immunoblot. *P < .05, n = 3 separate experiments. “Control” indicates “Control group without bacterial treatment”. (C) Wnt1 protein expression in Caco-2 cells. Cells were incubated with SL1314 for 30 minutes, washed, and incubated in fresh DMEM with 10% FBS for 30 minutes. Total cell lysates were analyzed for total Wnt1 levels by immunoblot. *P < .05, n = 3 separate experiments. (D) Salmonella treatment did not alter Wnt1 mRNA levels in HCT116 cells.