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Wnt1 reduction by Salmonella occurs through ubiquitination.
(A) Ubiquitination of Wnt1 increased in Salmonella-colonized cells by using immunoprecipitation of anti-ubiquitin or anti-IgG (negative control) and Wnt1. (B) The proteasome inhibitor MG132 stabilized Wnt1 protein after Salmonella colonization. Cells treated with MG132 (40 μM) for 4 hours had increased levels of Wnt1 protein. The data are presented as the mean ± SD of three replicate experiments.
