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. 2018 Apr 5;20(5):499–509. doi: 10.1016/j.neo.2018.03.001

Figure 3.

Figure 3

Downregulation of Wnt1 expression in intestinal epithelial cells alters the inflammatory response.

(A) Design of CRISPR sgRNA sequences targeting the N-terminus of Wnt1. (B) Western blot analysis of wnt1+/− cells. A Wnt1-specific antibody was used to detect Wnt1 protein in HCT116 lysates. (C) IL-8, IL-6, and GM-CSF mRNA levels in Wnt1-knockdown HCT116 cells after Salmonella colonization. (D-F) IL-8, IL-6, and GM-CSF mRNA levels in Wnt1-overexpressing HCT116 cells after Salmonella colonization. The data are presented as the mean ± SD of three replicate experiments.