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. 2018 Apr 5;20(5):499–509. doi: 10.1016/j.neo.2018.03.001

Figure 4.

Figure 4

Wnt1 expression in intestinal epithelial cells changed bacterial invasion.

(A) Cells with Wnt1 knockdown were resistant to invasion by GFP-Salmonella (green). (B) Number of invaded Salmonella in HCT116 cells with downregulation of Wnt1 expression compared to control group. (C) Number of Salmonella associated with HCT116 cells with/without knockdown of Wnt1. HCT116 cells with control RNAi and Wnt1knockdown cells were stimulated with Salmonella for 0.5 hour, respectively, and washed for the cell-associated bacteria; 0.9 ml LB broth was added; and each sample was mixed vigorously and quantified by plating for CFUs on MacConkey agar medium. The mean ± SD is from three replicate experiments. (D) Wnt1-overexpressing cells were sensitive to invasion by GFP-Salmonella (green). (E) Number of invaded Salmonella in HCT116 cells with overexpression of Wnt1 expression compared to control group. (F) Number of Salmonella associated with HCT116 cells with/without overexpression of Wnt1. The data are presented as the mean ± SD of three replicate experiments.