Fig. 1. AMC makes a break exclusively on target DNA. (a) Representative sequence/structure of DNA aligner (Left). The denaturing PAGE image shows that only the DNA aligner with one extra base pair beyond the recognition site can induce cleavage (Right). (b) Schematics of DNA complexes formed by fluorescently labelled target DNA and aligners (Top). The denaturing PAGE image shows that aligner-mediated cleavage exclusively occurs on target DNA (Red triangle), rather than on DNA aligner (Black triangle) (Bottom). (c) Schematics of DNA complexes formed by dual-labelled (Dabcyl and FAM) aligner or target sequence (Top). Real-time fluorescence upon adding Nt.BstNBI at 55 °C also shows a break of target sequence but not DNA aligner (Bottom).
